Le. Rikans et al., ALLYL ALCOHOL CYTOTOXICITY IN ISOLATED RAT HEPATOCYTES - EFFECTS OF AZIDE, FASTING, AND FRUCTOSE, Journal of toxicology and environmental health, 44(1), 1995, pp. 1-11
The role of altered energy homeostasis in the lethality of allyl alcoh
ol to isolated rat hepatocytes was studied. ATP, ADP, AMP, and viabili
ty loss (leakage of lactate dehydrogenase into the medium) were measur
ed in isolated hepatocytes of fed or fasted rats exposed to 0.5 mM all
yl alcohol. Adenine mononucleotides and cytotoxicity were determined a
lso in hepatocytes incubated with allyl alcohol in the presence of 4 m
M sodium azide or 15 mM fructose. Allyl alcohol-induced cell death in
hepatocytes of fed rats was preceded by slight decreases in ATP conten
t and energy charge (16% and 12%, respectively). More substantial decr
eases in these parameters occurred in parallel with cell killing, but
the effect of allyl alcohol on energy status did not exceed the effect
produced by a nonlethal concentration of sodium azide. Neither azide
nor fructose affected the development of allyl alcohol cytotoxicity. M
oreover, allyl alcohol-induced cytotoxicity was similar in hepatocytes
of fed and fasted rats. The results suggest that altered energy homeo
stasis is a consequence rather than a cause of allyl alcohol-induced h
epatocyte lethality.