Ja. Santiago et al., COMPARISON OF RESPONSES TO ADRENOMEDULLIN AND ADRENOMEDULLIN ANALOGS IN THE MESENTERIC VASCULAR BED OF THE CAT, European journal of pharmacology, 272(1), 1995, pp. 115-118
Responses to adrenomedullin, a newly discovered hypotensive peptide is
olated from human pheochromocytoma cells, and the carboxy terminal 15-
52 (adrenomedullin-(15-52)) and 22-52 (adrenomedullin-(22-52)) amino a
cid fragments of adrenomedullin were investigated in the mesenteric va
scular bed of the cat. Under constant flow conditions, injections of a
drenomedullin, adrenomedullin-(15-52), and calcitonin gene-related pep
tide (CGRP) in doses of 0.003-1 nmol into the perfused superior mesent
eric artery caused significant dose-related decreases in mesenteric ar
terial perfusion pressure. Mesenteric vasodilator responses to adrenom
edullin and adrenomedullin-(15-52) were similar in magnitude and durat
ion, while vasodilator responses to CGRP were greater in magnitude and
longer in duration than those produced by adrenomedullin or adrenomed
ullin-(15-52) when these agents were injected in doses of 0.1-1 nmol.
Adrenomedullin-(22-52) caused no significant change in mesenteric arte
rial perfusion pressure when injected in doses up to 10 nmol. These re
sults suggest that amino acids 15-52 and the six-membered ring structu
re of adrenomedullin are important for the expression of vasodilator a
ctivity in the mesenteric vascular bed of the cat.