CLONE ARCHITECTURE AND GROWTH-CHARACTERIZATION IN CHEMORADIOSENSITIVE, CHEMORESISTANT, RADIORESISTANT AND CHEMORADIORESISTANT NEOPLASTIC CELL-LINES

The characterization of the cell growth and the cell clone architectur e of four cell variants of the MXT mouse mammary and the T24 and J82 h uman bladder cell lines were assessed by means of a specific software combining Voronoi paving technique and digital cell image analyses of Feulgen-stained nuclei. The present methodological approach enabled th e done structure of cell lines growing on histological slides and thei r proliferation to be assessed concomitantly. The four cell variants c orresponded to chemoradiosensitive (S), chemoresistant (CR), radioresi stant (R) and chemoradioresistant (RCR) variants. The data relating to clone structure analyses revealed that cell variants that underwent t he more important therapeutic aggression (RCR variants) prior to the e xperiments are those who showed the more anarchic clone kinetics. In s ome cases, the data about the growth curves are perfectly explained by the clone kinetics, while it is not the cause in other circumstances. In these last eases in which the discrepancy is observed between cell growth curves data and cell clone architecture ones, we think that it need more investigations about phenomenon that might be linked to cel lular death.