L. Vuillard et al., NON-DETERGENT SULFOBETAINES - A NEW CLASS OF MILD SOLUBILIZATION AGENTS FOR PROTEIN-PURIFICATION, Biochemical journal, 305, 1995, pp. 337-343
The action of non-detergent sulphobetaines (NDSBs) as new mild agents
for protein purification is described. The solubilization effects of n
on-detergent sulphobetaines are shown in different examples, all obtai
ned under non-denaturing conditions: (1) microsomal proteins extractio
n; (2) recovery after dialysis of nuclear proteins; (3) reduction of p
recipitation in isoelectric focusing experiments under non-denaturing
conditions; and (4) purification of a membrane-bound serine protease f
rom Plasmodium falciparum involved in erythrocyte invasion by malaria
merozoites. The absence of a significant denaturation effect induced b
y NDSBs is demonstrated by test on beta-galactosidase and alkaline pho
sphatase. A simple NDSB synthesis and some possible explanations of th
e action of NDSBs are also presented.