Mp. Scolnik et al., OCTREOTIDE INHIBITS IN-VITRO B-CELL DIFFERENTIATION TO PLASMA-CELL INDUCED BY PWM, International journal of immunopathology and pharmacology, 9(3), 1996, pp. 119-122
Human leukocytes produce and are able to respond to some hormones. The
aim of this study was to determine the effect of octreotide (a synthe
tic analogue of somatostatin) on B-cell differentation to plasma cells
. Cells were obtained from peripheral blood of normal subjects (n = 7)
by Ficoll-Hypaque gradient. Octreotide was used in concentrations bet
ween 0 and 100 ng/ml. Plasma cells were determined as hemolysis plaque
forming cells and also by immunoflurescence after 7 day-culture of mo
nonuclear cells with and without PWM. Octreotide produced a dose-depen
dent decrease (congruent to 60%) in B-cell differentiation induced by
PWM. This effect began with 10 ng/ml. These results show that octreoti
de is able to regulate the function of B cells.