STUDIES ON IN-VITRO METABOLISM OF 7-N,N-DIMETHYLAMINO-1,2,3,4,5-PENTATHIOCYCLOOCTANE BY RAT-LIVER MICROSOMES PRETREATED WITH PHENOBARBITAL

Polythiocycloalkane (PTCA), -N,N-dimethylamino-1,2,3,4,5-pentathiocycl ooctane, is an insecticide developed in China during the past 10 years . In vitro metabolism of PTCA by rat liver microsomes was studied. The metabolites were isolated and characterized by high-performance liqui d chromatography, gas chromatography-mass spectroscopy, and infrared s pectroscopy. The results were verified by comparison with synthesized standards, evisect (N,N-dimethyl-1,2,3-trithian-5-amine) and nereistox in (N,N-dimethyl-1,2-dithiolan-4-amine), or by biochemical analysis. T he main metabolites were evisect, nereistoxin, monomethyl nereistoxin, 2-methylaminopropane, and sulfur polymers. The process was catalyzed mainly by enzymatic reactions, such as desulfuration and demethylation . The in vitro metabolism of PTCA by rat liver microsomes pretreated w ith phenobarbital may be a detoxification process. (C) 1995 Academic P ress, Inc.