VITAMIN-E PREVENTS COLD WRAP RESTRAINT STRESS-INDUCED INTESTINAL FLUID TRANSPORT ALTERATIONS IN RATS

Psychological stress may alter gastrointestinal absorptive function by increasing the quantity of intestinal free radicals or by lowering en dogenous intestinal free radical scavenging capacity. Vitamin E has be en shown to be a potent endogenous antioxidant and free radical scaven ger under both physiological and pathological conditions. The purpose of this study was to determine whether cold wrap restraint stress alte red in vivo intestinal fluid absorption in rats, and whether vitamin E administration prior to the induction of cold wrap restraint stress c ould prevent such changes in intestinal secretion. Jejunal, ileal and colonic fluid and electrolyte transport rates were measured in vivo us ing an isolated loop technique. Cold wrap restraint stress reduced in vivo fluid absorption in the ileum and colon, but not in the jejunum. Administration of vitamin E prior to the cold wrap restraint stress pr ocedure completely prevented this alteration of ileal and colonic flui d absorption.