A GENOMIC REGION ENCODING STONEFISH (SYNANCEJA-HORRIDA) STONUSTOXIN BETA-SUBUNIT CONTAINS AN INTRON

Authors
GHADESSY FJ JEYASEELAN K CHUNG MCM KHOO HE YUEN R
Citation
Fj. Ghadessy et al., A GENOMIC REGION ENCODING STONEFISH (SYNANCEJA-HORRIDA) STONUSTOXIN BETA-SUBUNIT CONTAINS AN INTRON, Toxicon, 32(12), 1994, pp. 1684-1688
Citations number
11
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Toxicon → ACNP
ISSN journal
00410101
Volume
32
Issue
12
Year of publication
1994
Pages
1684 - 1688
Database
ISI
SICI code
0041-0101(1994)32:12<1684:AGRES(>2.0.ZU;2-K
Abstract
The polymerase chain reaction (PCR) has been used to amplify a 1899 ba se pair fragment from stonefish genomic DNA. A comparison of the trans lated nucleotide sequence of this product with the separately determin ed N-terminal amino acid sequence of the beta-subunit reveals the pres ence of a 416 bp intron at Gly 18. The nucleotide sequence following t his intron encodes 476 amino acids whose sequence showed no homology t o other known toxins. This region, however, contained amino acid seque nces identical to internal peptide sequences determined separately fro m the toxin's beta-subunit.