Aging is associated with disturbances in autonomic cardiovascular cont
rol.(5,19) The purpose of this study was to test the hypothesis that c
hanges in nitric oxide occur with aging in brainstem nuclei involved i
n autonomic cardiovascular control, The principal and unexpected findi
ng in this study was that NADPH-diaphorase reactivity, which is consid
ered a marker of neuronal nitric oxide synthase activity,(7,8) was dec
idedly increased in the neuronal bodies of the gracile nucleus but dec
reased in the axons and axon terminals in old compared with young rats
, In situ hybridization also revealed that nitric oxide synthase gene
expression was enhanced predominantly in the gracile nucleus neurons o
f aged rats, The differences between the young and old rats were most
dramatically evident in the gracile nucleus, but not evident in other
brainstem nuclei, The significance of this finding as it might relate
to autonomic or other specific neural dysfunction with aging is not ev
ident at this time. Copyright (C) 1996 IBRO.