PEANUT PEROXIDASE, ITS LOCATION AND EXTENSIN, CONIFERYL OXIDATION

Antibodies, raised against highly purified peanut (Arachis hypogaea) p eroxidase (EC 1.11.1.7) isozymes, indicated through immune-gold labell ing that the cationic isozyme occurs mainly in the cell wall and the a nionic one in the cytoplasm. Extensin incubated with the cationic pero xidase and examined by electron microscopy showed linkages. By thin la yer chromatography separation of the hydrolyzates of the incubation, i sodityrosine residues were detected. The results confirm that peroxida se mediates tyrosine linkages in extensin, In terms of the binding of coniferyl alcohol both. isozymes were equally capable to mediate bindi ng. The greatest amount of binding of coniferyl alcohol occurred to po ly-galacturonic acid.