FROM CLONES OF CELLS TO CLONES OF GENES

Three approaches to study the immune system are presented. First is th e limiting dilution analysis, second is the analysis of the polypeptid e patterns of lymphocytes by two-dimensional gel electrophoresis, and the third is the utilization of a partitioned cDNA library for establi shing a gene catalog and proteinpaedia. All the approaches were used t o study clonal diversity and clonal heterogeneity. The gene catalog wh ich is already established is available to recover genes expressed in various lymphocyte clones.