EPITOPE ANALYSIS USING ANTI-OLIGOSACCHARIDE (G4) MONOCLONAL-ANTIBODY

Authors
HIRATA A KOMODA M ITOH W TABATA K SUGAWARA I
Citation
A. Hirata et al., EPITOPE ANALYSIS USING ANTI-OLIGOSACCHARIDE (G4) MONOCLONAL-ANTIBODY, Microbiology and immunology, 39(1), 1995, pp. 43-47
Citations number
13
Categorie Soggetti
Microbiology,Immunology
Journal title
Microbiology and immunology → ACNP
ISSN journal
03855600
Volume
39
Issue
1
Year of publication
1995
Pages
43 - 47
Database
ISI
SICI code
0385-5600(1995)39:1<43:EAUA(M>2.0.ZU;2-6
Abstract
A murine monoclonal antibody recognizing (1 --> 6)-beta-D-glucopyranos yl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4- bovine serum albumin conjugate and fusing the splenoclytes with mouse myeloma cells, The monoclonal antibody (IgM) provoked by the cloned ce lls showed low reactivity with schizophyllan, an antitumor polysacchar ide, but notable reactivity with some low-molecular-weight schizophyll ans. This antibody was useful for determination of the epitope of seve ral polysaccharides. The extent of reactivity of this monoclonal antib ody was related only to the molecular weight of schizophyllan.