A murine monoclonal antibody recognizing (1 --> 6)-beta-D-glucopyranos
yl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-
bovine serum albumin conjugate and fusing the splenoclytes with mouse
myeloma cells, The monoclonal antibody (IgM) provoked by the cloned ce
lls showed low reactivity with schizophyllan, an antitumor polysacchar
ide, but notable reactivity with some low-molecular-weight schizophyll
ans. This antibody was useful for determination of the epitope of seve
ral polysaccharides. The extent of reactivity of this monoclonal antib
ody was related only to the molecular weight of schizophyllan.