MOLECULAR MARKERS NEAR 2 MOUSE CHROMOSOME-13 GENES, MUTED AND PEARL, WHICH CAUSE PLATELET STORAGE POOL DEFICIENCY (SPD)

The recessive muted (mu) and pearl (pe) mutations on Chromosome (Chr) 13 cause pigment dilution and platelet storage pool deficiency (SPD) i n mice. In addition, mu causes inner ear abnormalities and pe has symp toms associated with night blindness. Using an interspecific backcross involving the wild-derived Mus musculus musculus (PWK) stock, we have mapped 33 microsatellite markers and four cDNAs relative to mu, pe, a nd another recessive mutation, satin (sa). Analyzing a total of 528 ba ckcross offspring, we found tight linkage between the pigment loci and several microsatellite markers (D13Mit87, D13Mit88, D13Mit137 with mu ; and D13Mit104, D13Mit160, D13Mit161, and D13Mit169 with pe). These m arkers should aid the eventual molecular identification of these speci fic SPD genes.