T. Leeb et al., CONSTRUCTION OF A PORCINE YAC LIBRARY AND MAPPING OF THE CARDIAC-MUSCLE RYANODINE RECEPTOR GENE TO CHROMOSOME 14Q22-Q23, Mammalian genome, 6(1), 1995, pp. 37-41
Large-scale physical mapping of the porcine genome has been limited be
cause up to now no suitable genomic libraries for this purpose have be
en available. Therefore, we have constructed a yeast artificial chromo
some (YAC) library from porcine lymphocytes. The library was cloned in
the amplifiable vector pCGS966. A total of 10080 YAC clones was obtai
ned and has been ordered into 105 96-well microtiter plates. An averag
e insert size of 300 kb was calculated from the analysis of 78 randoml
y selected clones, giving onefold coverage of the porcine genome. To a
nalyze the complexity, we have screened the library for five different
genes and isolated four different clones containing parts of three of
these genes. One YAC clone harboring parts of the porcine cardiac mus
cle ryanodine receptor (RYR2) gene allowed us to assign this locus to
Chromosome (Chr) 14q22-q23. The data were confirmed by PCR analysis of
a rodent-porcine hybrid cell panel.