CONSTRUCTION OF A PORCINE YAC LIBRARY AND MAPPING OF THE CARDIAC-MUSCLE RYANODINE RECEPTOR GENE TO CHROMOSOME 14Q22-Q23

Large-scale physical mapping of the porcine genome has been limited be cause up to now no suitable genomic libraries for this purpose have be en available. Therefore, we have constructed a yeast artificial chromo some (YAC) library from porcine lymphocytes. The library was cloned in the amplifiable vector pCGS966. A total of 10080 YAC clones was obtai ned and has been ordered into 105 96-well microtiter plates. An averag e insert size of 300 kb was calculated from the analysis of 78 randoml y selected clones, giving onefold coverage of the porcine genome. To a nalyze the complexity, we have screened the library for five different genes and isolated four different clones containing parts of three of these genes. One YAC clone harboring parts of the porcine cardiac mus cle ryanodine receptor (RYR2) gene allowed us to assign this locus to Chromosome (Chr) 14q22-q23. The data were confirmed by PCR analysis of a rodent-porcine hybrid cell panel.