SIMULTANEOUS DETERMINATION OF FREE AND BOUND ADRIAMYCIN, ADRIAMYCINOL, ADRIAMYCINONE AND DUANORUBICIN IN PLASMA USING COLUMN-SWITCHING TECHNIQUE AND PROTEIN-COATED PRE-COLUMNS

Adriamycin, adriamycinol, adriamycinone and duanorubicin were simultan eously determined by the development of an on-line plasma clean-up sys tem. A short protein-coated Lichrosorb, RP-8, RP-2, CN and mu Bondapak phenyl as well as ODS silica have been examined for their performance as pre-columns. The drugs and metabolites were separated from weakly retained plasma components through two steps; phosphate buffer saline, pH 7.4 and 15% acetonitrile in 0.1M sodium dihydrogen phosphate, pH 3 . The chromatographic conditions were: ODS/TM column, flow rate 1 ml/m in, 35% acetonitrile in 0.1M sodium dihydrogen phosphate (pH 3) contai ning 0.3% heptafluorobutyric acid as mobile phase. The detection was c arried out using fluorescence monitor operated at an emission 555 nm a nd excitation 460 nm. Good resolution was obtained within 13 min. This method is reproducible for analysis of drugs and metabolites (99.3-10 0.1%, CV <2%) in plasma.