EXPRESSION OF ALPHA-AMYLASE GENE OF BACILLUS-LICHENIFORMIS IN SACCHAROMYCES-CEREVISIAE

Authors
LUO JX LI Y LI WQ ZHANG TY HE M
Citation
Jx. Luo et al., EXPRESSION OF ALPHA-AMYLASE GENE OF BACILLUS-LICHENIFORMIS IN SACCHAROMYCES-CEREVISIAE, Science in China. Series B, Chemistry, life sciences & earth sciences, 37(11), 1994, pp. 1321-1328
Citations number
15
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Science in China. Series B, Chemistry, life sciences & earth sciences → ACNP
ISSN journal
1001652X
Volume
37
Issue
11
Year of publication
1994
Pages
1321 - 1328
Database
ISI
SICI code
1001-652X(1994)37:11<1321:EOAGOB>2.0.ZU;2-A
Abstract
The secretive expression vector has been constructed using the promote r and signal sequence of yeast MF-alpha 1 factor, and the Bacillus lic heniformis alpha-amylase gene without promoter and signal sequence has been inserted into the downstream of the signal sequence on the vecto r. After the readjustment of the reading frame, the amylase gene was e xpressed in Saccharomyces cerevisiae and the product was secreted from it. The properties of enzymes secreted from yeast and B. subtilis are compared, and the mechanism of the gene expression and product secret ion are discussed.