CAMPYLOBACTER HYOILEI SP-NOV, ASSOCIATED WITH PORCINE PROLIFERATIVE ENTERITIS

Campylobacter hyoilei sp, nov, is the name proposed for an organism fo rmerly described as strain RMIT 32A(T) (T = type strain) and a group o f similar bacteria isolated from intestinal lesions of pigs with proli ferative enteritis. The phenotypic characteristics of these organisms indicated that they are closely related to each other and are not stra ins of other Campylobacter spp, commonly isolated from pigs, The resul ts of probing of ClaI-, EcoRV-, or BglII-cleaved genomic DNAs from C. hyoilei strains with a radiolabeled DNA probe that distinguishes betwe en Campylobacter jejuni and Campylobacter coli indicated that C, hyoil ei and C, coli are closely related, However, the 16S rRNA sequence of the reference strain of C. hyoilei, RMIT 32A(T), was four bases differ ent from the 16S rRNA sequence of C. jejuni CCUG 11284(T) and five bas es different from the 16S rRNA sequence of C. jejuni subsp. doylei CCU G 24567(T), suggesting that C, hyoilei is more closely related to C. j ejuni than to C. coli. Hybridization between DNA from C, hyoilei type strain RMIT 32A and DNAs from selected type and reference strains of o ther Campylobacter species and subspecies, including C. jejuni, C. jej uni subsp. doylei, C. coli, Campylobacter mucosalis, and Campylobacter hyointestinalis, as well as the other C. hyoilei strains (the RMIT 32 A(T)-like isolates), revealed that high levels of DNA hybridization (> 70%) occurred only between the reference strain and other strains of C . hyoilei.