CHARACTERISTICS OF RHIZOBIUM-TIANSHANENSE SP-NOV, A MODERATELY AND SLOWLY GROWING ROOT-NODULE BACTERIUM ISOLATED FROM AN ARID SALINE ENVIRONMENT IN XINJIANG, PEOPLES-REPUBLIC-OF-CHINA

We performed a numerical analysis of 148 phenotypic characteristics of 20 strains of root nodule bacteria isolated from an arid saline deser t soil in the Xinjiang region of northwestern People's Republic of Chi na and compared these organisms with 28 Rhizobium and Bradyrhizobium s trains obtained from different regions of the People's Republic of Chi na and from other countries, including nine type strains of different species. All of the strains examined clustered into two groups at a si milarity level of more than 63%. Group I included all of the previousl y described Rhizobium species and was divided into eight subgroups, wh ich corresponded to previously described Rhizobium species, at a simil arity level of more than 82%. Group II was divided into the following three subgroups at a similarity level of more than 80% Bradyrhizobium japonicum, a cluster containing 17 moderately and slowly growing strai ns isolated in the Xinjiang region, and a small subgroup containing th ree fast-growing strains. The generation times of the moderately and s lowly growing strains were 5 to 15 h, and these organisms produced aci d in medium containing mannitol. The DNA G+C contents of the members o f this group ranged from 59 to 63 mol%. DNA-DNA hybridization experime nts revealed that the levels of DNA homology among all of the moderate ly-and slowly growing strains obtained from Xinjiang were more than 70 % and that the levels of DNA homology between representative strains o f this group and the type strains of all previously described species of root- and stem-nodulating bacteria were low. All of our experimenta l data indicated that the moderately and slowly growing rhizobia isola ted from Xinjiang are members of a new species. Partial 168 rRNA gene sequencing of the type strain. A-1BS (= CCBAU3306), and a comparison o f the resulting sequence with the sequences of previously described sp ecies revealed that strain A-1BS is closely related to Rhizobium loti, Rhizobium huakuii, and Rhizobium galegae, but not to B. japonicum and Azorhizobium caulinodans. On the basis of our results, we propose tha t the strains which we studied are members of a new species, Rhizobium tianshanense. The type strain, A-1BS (= CCBAU3306), has been deposite d in the Culture Collection of Beijing Agricultural University, Beijin g, People's Republic of China.