OZONE-INDUCED METABOLIC CHANGES IN SCOTS PINE - MESSENGER-RNA ISOLATION AND ANALYSIS OF IN-VITRO TRANSLATED PROTEINS

A rapid isolation procedure for intact poly(A)(+) RNA from Scots pine (Pinus sylvestris L.) is described. Translation products of poly(A)(+) RNA isolated from 6-week-old Scots pine seedlings treated with ozone (0.3 mu L/L) for 8 h were analyzed using two-dimensional polyacrylamid e gel electrophoresis (2D-PAGE). Both qualitative and quantitative dif ferences in the translation products were observed after ozone fumigat ion. Expression of specific mRNAs, coding for stress-induced polypepti des between 12 kD and 50 kD, was demonstrated after 8 h of ozone treat ment.