SIMULTANEOUS DETERMINATION OF PYRIDOXAL AND PYRIDOXAMINE BY DIFFERENTSPECTROFLUOROMETRIC TECHNIQUES

Binary mixtures of pyridoxal and pyridoxamine can be resolved by using zero-crossing first derivative spectrofluorimetry, first derivative c onstant wavelength synchronous luminescence spectrometry and first der ivative constant energy synchronous luminescence spectrometry. These m ethods do not require any previous separation steps. The lowest quanti zation limit is obtained with first derivative constant energy synchro nous fluorescence (13.0 and 9.0 mu g/l. for pyridoxal and pyridoxamine , respectively). The measurements were performed in aqueous medium at pH 7.0 provided by adding 0.05M phosphate buffer solution. In order to demonstrate the validity of these methods a complete and exhaustive s tatistical analysis of the experimental data was performed. Pyridoxal and pyridoxamine were determined by these methods in synthetic and rea l mixtures with good results.