MONOCLONAL-ANTIBODY AGAINST HUMAN GLIOBLASTOMA-MULTIFORME (U-87MG) IMMUNOPRECIPITATES A PROTEIN OF MOLECULAR-MASS 38-KDA AND INHIBITS TUMOR-GROWTH IN NUDE-MICE

A monoclonal antibody 6DS(1) against a human glioblastoma multiforme c ell line U-87MG recognizes a tumor-specific, cell surface antigen of h uman glioblastoma cell lines. Partial cross-reactivity is observed wit h two human neuroblastoma cell lines, SK-N-SH and SK-N-MC, with little or no reactivity towards a rat glioma cell line C6 or normal human ad ult and fetal brain tissues. The antibody recognizes an antigen of mol ecular mass 38 kDa as inferred from Western blot analysis and sodium d odecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipi tate. The monoclonal antibody 6DS(1) inhibits both the attachment to s ubstratum and growth of U-87MG cells. It strongly cross-reacts with xe notransplants of U-87MG cells and inhibits tumorigenesis (subcutaneous implants of U-87MG cells) in nude mice.