QUANTITATIVE COMPUTERIZED ANALYSIS OF SILVER-STAINED AND COOMASSIE BLUE-STAINED 2-DIMENSIONAL PROTEIN MAPS

Using the two-dimensional gel electrophoresis and two systems for comp uter analysis of the resulting maps, we obtained a good quantitative r eproducibility of individual spots of silver-stained and Coomassie Blu e-stained gels from mouse fetus liver samples. The GelScan-XL system w as established for the evaluation of silver-stained gels. The Gel-Imag e software has shown to be capable of the analysis of Coomassie Blue-s tained gels and autoradiographs. For these systems the linear relation ship between defined concentrations of protein loaded on the gel and t he resulting integrated intensities of spots were examined. The result s show that both systems are efficient to detect quantitative changes in protein expression correlated with malformations in mouse fetuses.