STUDIES OF SOME EXPERIMENTAL CONDITIONS FOR THE POLYMERIZATION OF THECONTINUOUS BEDS (CROSS-LINKED POLYACRYLAMIDE GELS) IN ORDER TO IMPROVE THEIR CHROMATOGRAPHIC PROPERTIES

Continuous chromatographic beds are synthesized by a simple and cost-e ffective procedure which does not involve the conventional, time-consu ming preparation of beads. The aim of this study was to improve the fl ow properties of the beds by investigating how they are affected by po lymerization temperature, the pH of the buffer in which the polymeriza tion takes place, the nature of the constituents of this buffer, the c oncentrations of catalyst and accelerator and the concentration of the precipitating agent (ammonium sulfate). The influence of flow rate an d the time constants of the detector and recorder on peak width was al so studied.