HIGH-SPEED CHIRAL SEPARATION OF BETA-BLOCKERS BY SUPERCRITICAL-FLUID CHROMATOGRAPHY ON CHYROSINE-A

High-speed separation of beta-blockers have been successfully obtained (analysis time <2 min) using a short column packed with ChyRoSine-A C SP. Peculiar chromatographic behaviour of beta-blockers on tyrosine-de rived CSPs under supercritical. conditions was evidenced by studying t he curves of h versus the linear velocity u. Specific solvation of sol utes by supercritical carbon dioxide is responsible for the particular conformation of the molecule of beta-blocker which enhances chiral di scrimination. This was evidenced by using the reciprocality concept le ading to a new CSP derived from a propranolol analogue. Thus, it was d emonstrated that SFC can be used for the fast chiral separation of bet a-blockers.