EPIBRASSINOLIDE EFFECT ON ATPASE ACTIVITY OF PLASMALEMMA AND OTHER CELL COMPONENTS FROM BUCKWHEAT SEEDLINGS OF A DIFFERENT PLOIDY LEVEL

As was revealed by biochemical methods under in vitro conditions epibr assinolide (10(-7) M) increased ATPase activity of plasma membranes is olated in sucrose isopicnic density gradient from tetraploid buckwheat (Fagopyrum esculentum Moench.) seedlings and did not change it in dip loid ones within 30 min of exposure. Under in vivo conditions epibrass inolide (10(-7) M, 30 min) inhibited ATPase activity of plasma membran es irrespective of buckwheat seedling ploidy. The total ATPase activit y increased by 35% in tetraploid seedlings and decreased twice in dipl oid ones. It was due to similar changes in the activity of ATPases tha t were not inhibited by uabain and orthovanadate.