STRUCTURE OF THE GENE ENCODING PIG PHOSPHOGLUCOSE ISOMERASE

Genomic clones encoding pig phosphoglucose isomerase (PGI) have been i solated and partially sequenced. The gene (Pgi) contains 18 exons, 17 introns and spans about 32 kb. This structure is partially conserved b etween plant and animal. A major transcription start point (tsp) has b een identified 74 nucleotides (nt) upstream from the AUG. The nt seque nce around the tsp is very G+C rich; a 5'-ATAAA sequence, as well as f our putative Sp1-binding sites, are present. In the 3'-flanking region , an AATAAA signal has been identified. Extending from the 5'-flanking region to the first intron, a 0.6-kb CpG island has been identified. The Pgi structural gene contains several DNA repetitive elements in it s non-coding regions.