CLONING OF CDNAS CODING FOR THE G-ALPHA-I1 AND G-ALPHA-I2 G-PROTEINS FROM CHICK BRAIN

We have cloned and characterized several cDNAs coding for G-protein in hibitory alpha subunits (G alpha i) from a chick brain cDNA library. B ased on homology to G alpha subunits from other eukaryotes, these clon es were designated chick G alpha i1 and G alpha i2. On the deduced ami no-acid level, G alpha i1 and G alpha i2 were found to be 98 and 95% i dentical to rat G alpha i1 and G alpha i2, respectively. Using RNase p rotection analysis, the G alpha i1 and G alpha i2 mRNAs were found to be expressed in chick atria, ventricle, lung, liver, brain and kidney.