P. Garcialopez et al., COMPARISON BETWEEN SPRAGUE-DAWLEY AND WISTAR RATS AS AN EXPERIMENTAL-MODEL OF PHARMACOKINETIC ALTERATIONS INDUCED BY SPINAL-CORD INJURY, Archives of medical research, 27(4), 1996, pp. 453-457
Two strains of rats, Sprague-Dawley and Wistar, were assayed in order
to determine which strain is the more suitable experimental model for
the study of pharmacokinetic alterations induced by spinal cord injury
. Animals were submitted to spinal cord contusion at the T8-T9 level b
y the weight drop method. A single acetaminophen oral dose (100 mg/kg)
was administered 24 h after injury and blood samples were drawn for a
period of 4 h. Acetaminophen concentration in whole blood was determi
ned by high performance liquid chromatography and pharmacokinetic para
meters were estimated. For both strains, C-max and AUC were significan
tly lower, whereas t(max) remained unchanged, in injured animals compa
red to sham-injured controls. Circulating acetaminophen concentrations
were higher; therefore, pharmacokinetic alterations were more easily
discerned, in Sprague-Dawley than in Wistar rats. It is concluded that
the Sprague-Dawley strain is a more suitable model for the study of p
harmacokinetic alterations induced by spinal cord injury.