PREGNANCY OBTAINED WITH HUMAN TESTICULAR SPERMATOZOA IN AN IN-VITRO FERTILIZATION PROGRAM

The fertilizing capacity of human testicular spermatozoa and the posit ive outcome of an in vitro fertilization program open a wide range of opportunities for men suffering from obstructive and inoperable azoosp ermia. In six cases, subzonal sperm injection or intracytoplasmic sper m injection techniques were applied to inject testicular spermatozoa i nto human oocytes. The fertilization rate after testicular sperm injec tion reached 45%. Normal cleavage was observed and replacement of 10 e mbryos in 6 patients resulted in one chemical and one ongoing pregnanc y.