J. Castrogarza et al., COMPARABLE GROWTH OF A TRICHOMONAS-VAGINALIS STRAIN IN PEHPS AND TYI-S-33 MEDIA, Archives of medical research, 27(4), 1996, pp. 567-569
PEHPS medium, developed for axenic cultivation of I Entamoeba histolyt
ica and E. invadens, was also capable of supporting the growth of a Tr
ichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/
ml. The logarithmic growth phase in PEWS or in TYI-S-33 medium lasted
72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time
(4.27 h), number of duplications (16.85), or increase ratio (33,328) i
n PEHPS medium showed no significant differences with those obtained i
n TYI-S-33 under similar culture conditions. Accordingly, PEHPS medium
might be used for the axenic cultivation of T. vaginalis.