Cell wall ultrastructure of the opportunistic pathogenic yeast Candida
albicans was investigated by stereoscopic freeze-etching technique. T
hree wall layers were distinguishable by this technique. No clear peri
plasmic space was evident. Bilayer membrane invaginations were extensi
ve. The outermost regions of the membrane invaginations were lined wit
h thin, spine-like fibrils, which extended into the cell wall. We sugg
est that the fibrils along the invaginations are involved in anchoring
the cell wall to the membrane.