AN INTRON-CONTAINING TRNA(ARG) GENE WITHIN A LARGE CLUSTER OF HUMAN TRANSFER-RNA GENES

The insert within lambda Ht363, a recombinant selected from a bank of human genomic DNA cloned in lambda Ch4A, is described. Southern blot h ybridization with a mixed tRNA[P-32]pCp probe revealed the presence of four tRNA genes, which were shown to represent further copies of gene s previously identified as a solitary tRNA(Gly) gene and as a three ge ne cluster on two different recombinants. In vitro transcription of a fragment containing the three gene cluster revealed the presence of a further pol III gene, which was shown to be that for a tRNA(TCT)(Arg). This gene contains a 15 bp intron, the presence of which presumably p re vented its detection on Southern blots by tRNA hybridisation. The g ene is present in the previously reported cluster and occurs in higher copy number (>7) in other arrangements in the genome. Most of the cop ies of the gene have related intron sequences.