COMPARISON OF GONOGEN, GONOGEN-II, AND MICROTRAK DIRECT FLUORESCENT-ANTIBODY TEST WITH CARBOHYDRATE FERMENTATION FOR CONFIRMATION OF CULTURE ISOLATES OF NEISSERIA-GONORRHOEAE
Ja. Kellogg et Lk. Orwig, COMPARISON OF GONOGEN, GONOGEN-II, AND MICROTRAK DIRECT FLUORESCENT-ANTIBODY TEST WITH CARBOHYDRATE FERMENTATION FOR CONFIRMATION OF CULTURE ISOLATES OF NEISSERIA-GONORRHOEAE, Journal of clinical microbiology, 33(2), 1995, pp. 474-476
When testing 248 clinical isolates of Neisseria gonorrhoeae, the sensi
tivity was 100% with GonoGen (Becton Dickinson Microbiology Systems),
99.6% (247 of 248) with GonoCen II (Becton Dickinson), 97.2% (241 of 2
48) with the MicroTrak direct fluorescent-antibody test (Syva), and 97
.6% (242 of 248) with Rapid Fermentation Agar carbohydrates (Remel). O
f 62 isolates of other Neisseria species, none was misidentified as N.
gonorrhoeae by GonoGen, MicroTrak, or Rapid Fermentation Agar carbohy
drates but 7 (31.8%) of 22 isolates of N. meningitidis gave strong, re
peatedly false-positive results with GonoGen II. The sensitivity of al
l four assays was good to excellent, but all positive GonoGen II resul
ts should be confirmed with an independent assay, especially when isol
ates are recovered from sites where N. meningitidis is likely. Positiv
e results from any of the assays should be routinely confirmed when di
ctated by specific clinical, legal, or microbiological circumstances.