SPECIFIC AMPLIFICATION OF RICKETTSIA-JAPONICA DNA FROM CLINICAL SPECIMENS BY PCR

Authors
FURUYA Y KATAYAMA T YOSHIDA Y KAIHO I
Citation
Y. Furuya et al., SPECIFIC AMPLIFICATION OF RICKETTSIA-JAPONICA DNA FROM CLINICAL SPECIMENS BY PCR, Journal of clinical microbiology, 33(2), 1995, pp. 487-489
Citations number
14
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
2
Year of publication
1995
Pages
487 - 489
Database
ISI
SICI code
0095-1137(1995)33:2<487:SAORDF>2.0.ZU;2-W
Abstract
The gene encoding the 17,000-molecular-weight genus-common antigen (17 K genus-common antigen) has been cloned and sequenced from Rickettsia japonica. The primer pair used for PCR was designed from this sequence . A 357-bp fragment was observed by amplifying the genomic DNA from R. japonica and also the DNA from blood clots of patients with spotted f ever group rickettsiosis. The results indicated that this method is su itable for the diagnosis of spotted fever group rickettsiosis in Japan .