AUTOANTIBODIES-LIKE ANTIGEN-BINDING CHARACTERISTICS OF INDUCED ANTIBODIES AGAINST POLYLYSINE-POLYGLUTAMATE COMPLEX

Rabbits immunized with polylysine-polyglutamate complex induced high t iter antibodies. The immune IgG exhibited high degree of specificity t owards the immunogen. In a competition assay, nDNA, heat denatured DNA and total buffalo thymus RNA were inhibitory. An appreciable binding with polyglutamate, poly(rG).poly(dC).poly(dG).poly(dC), cardiolipin, poly(dA-dU). Poly(dA-dU) and Z-or Z-like conformations demonstrates th e polyspecificity of induced antibodies. The immunological specificity of induced antibodies was comparable to autoantibodies derived from S LE. The results might be of some help in understanding the etiology of autoimmune process.