Gn. Fomovskaya et al., EXPRESSION OF SOME ONCOFETAL ANTIGENS IN PERIPHERAL MONONUCLEAR-CELLSOF HUMAN AND MONKEY EXPOSED TO LOW-DOSES OF IONIZING-RADIATION, Eksperimental'naa onkologia, 16(2-3), 1994, pp. 174-180
Expression of carcinoembryonic antigen (CEA), trophoblast-specific bet
a1-glycoprotein (TSG) and embryonic prealbumin-1 (EPA) on peripheral b
lood mononuclear cells (PBM) was studied in the blood of: a) healthy d
onors, inhabitants of Kiev, b) persons who participated in the reconst
ruction of Chernobyl AS and obtained the doses of combined irradiation
up to 0.25 Gy and c) Pavian-gamadril monkeys exposed to the doses of
0.125; 0.250; 0.675; 1.00 Gy. The study was carried out by means of fl
ow cytometry with anti-CEA, anti-TSG and anti-EPA rabbit antibodies an
d FITC-conjugated donkey anti-rabbit antibodies. 3-20% of PBM of healt
hy donors bound Abs specific to CEA, TSG and EPA. Pretreatment with 0.
05 glycine buffer containing 0.14 M NaCl, pH=2.8, didn't influence the
PBM capacity to bind these Abs. Persons who participated in the recon
struction of Chernobyl AS had either PBM levels undistinguishable from
those of healthy donors or didn't have PBM binding anti-CEA, anti-TSG
and anti-EPA Abs at all. Content of PBM expressing CEA, TSG and EPA d
idn't exceed 10% for each antigen both in unirradiated monkeys and in
those after irradiation with 0.125 Gy. Further irradiation resulted in
dramatic increase of the studied cells number. In 1 and 2.5 months af
ter irradiation the content of PBM expressing these antigens in monkey
s irradiated with 1.0 Gy became comparable with that found in unirradi
ated animals.