MTT-ASSAY IN PREDICTION AND DETECTION OF REGULARITIES IN INDIVIDUAL CHEMOSENSITIVITY OF HUMAN OVARIAN CARCINOMAS

Individual chemosensitivity to different cytotoxic agents was examined in 53 samples of human ovarian carcinomas using the MTT-assay. Compar ison of the results obtained by the classic clonogenic assay and by th e MTT-assay proved adequacy of the latter for prediction of chemosensi tivity of ovarian carcinoma cells. Significant variability in chemosen sitivity of avarian carcinomas to the same drugs was detected. Using a statistical analisis of dose-effect curves (244 curves) four types of the dependence of malignant cells' destruction on the dose of cytosta tics have been found and, the types of dose-effect curves for each of the antitumour agents studied have been determined. Proceeding from th e data obtained a number of theses concerning improvement of the ovari an cancer treatment using relevant chemotherapy are formulated.