ESCHERICHIA-COLI DIHYDRODIPICOLINATE SYNTHASE - CHARACTERIZATION OF THE IMINE INTERMEDIATE AND THE PRODUCT OF BROMOPYRUVATE TREATMENT BY ELECTROSPRAY MASS-SPECTROMETRY

Authors
BORTHWICK EB CONNELL SJ TUDOR DW ROBINS DJ SHNEIER A ABELL C COGGINS JR
Citation
Eb. Borthwick et al., ESCHERICHIA-COLI DIHYDRODIPICOLINATE SYNTHASE - CHARACTERIZATION OF THE IMINE INTERMEDIATE AND THE PRODUCT OF BROMOPYRUVATE TREATMENT BY ELECTROSPRAY MASS-SPECTROMETRY, Biochemical journal, 305, 1995, pp. 521-524
Citations number
18
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
305
Year of publication
1995
Part
2
Pages
521 - 524
Database
ISI
SICI code
0264-6021(1995)305:<521:EDS-CO>2.0.ZU;2-N
Abstract
Simplified procedures for assaying and purifying dihydrodipicolinate s ynthase (EC 4.2.1.52), the first enzyme of the lysine biosynthetic pat hway, have been developed and electrospray ionization m.s. has been us ed to observe the imine intermediate with pyruvate and to investigate the reaction of the enzyme with bromopyruvate and fluoropyruvate.