EFFICIENT OPTIMIZATION OF ELISAS

Authors
REIKEN SR VANWIE BJ SUTISNA H KURDIKAR DL DAVIS WC
Citation
Sr. Reiken et al., EFFICIENT OPTIMIZATION OF ELISAS, Journal of immunological methods, 177(1-2), 1994, pp. 199-206
Citations number
5
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
177
Issue
1-2
Year of publication
1994
Pages
199 - 206
Database
ISI
SICI code
0022-1759(1994)177:1-2<199:EOOE>2.0.ZU;2-3
Abstract
In this paper, we describe a technique for using the statistical metho d of fractional factorial design in the optimization of an ELISA. Frac tional factorial design dramatically reduces the total number of exper iments required in the optimization. In addition, this technique enabl es us to determine the parameters that give the maximum sensitivity ra nge with the most accuracy for the ELISA studied.