We analysed 595 cows in 20 herds for serological evidence of paratuber
culosis with an ELISA procedure for which we had recently determined a
sensitivity of 50% and a specificity of 98%, and we obtained an overa
ll calculated real prevalence of 5,99%. In two herds with clincal case
s of paratuberculosis, the calculated true prevalence of seroreactors
was 21,47%. In 9 herds with clinical suspicion of Johne's disease, the
rate was 4,23%, 9 herds without any clinical suspicion showed a mean
prevalence of 0%. Whereas the trend was clear for groups of herds, the
observed prevalence in individual herds varied from 0% to 17,9%, with
out obvious association with manifest clinical problems of paratubercu
losis.