N. Bogdanova et al., CYSTEINE BIOSYNTHESIS IN PLANTS - ISOLATION AND FUNCTIONAL IDENTIFICATION OF A CDNA-ENCODING A SERINE ACETYLTRANSFERASE FROM ARABIDOPSIS-THALIANA, FEBS letters, 358(1), 1995, pp. 43-47
A cDNA encoding for serine acetyltransferase which catalyzes the commi
tting step of cysteine biosynthesis has been cloned from Arabidopsis t
haliana. The plant protein has a predicted molecular weight of 32.8 kD
a and shows up to 43% of amino acid homology to bacterial serine acety
ltransferases. It complements a serine acetyltransferase negative E. c
oli mutant and can be enzymatically determined in the heterologous hos
t. The corresponding mRNA is predominantely expressed in light exposed
tissue and represents one of at least two related genes.