AMPLIFICATION OF EXON-11 OF THE GENE FOR THE ALPHA-CHAIN OF BETA-N-ACETYLHEXOSAMINIDASE IN SINGLE HUMAN BLASTOMERES

Authors
SERMON K LISSENS W DEVROEY P VANSTEIRTEGHEM A LIEBAERS I
Citation
K. Sermon et al., AMPLIFICATION OF EXON-11 OF THE GENE FOR THE ALPHA-CHAIN OF BETA-N-ACETYLHEXOSAMINIDASE IN SINGLE HUMAN BLASTOMERES, Fertility and sterility, 63(2), 1995, pp. 407-409
Citations number
5
Categorie Soggetti
Obsetric & Gynecology
Journal title
Fertility and sterility → ACNP
ISSN journal
00150282
Volume
63
Issue
2
Year of publication
1995
Pages
407 - 409
Database
ISI
SICI code
0015-0282(1995)63:2<407:AOEOTG>2.0.ZU;2-N
Abstract
Objective: To evaluate the possibility of using polymerase chain react ion (PCR) technology for preimplantation diagnosis for Tay-Sachs disea se. Design: Polymerase chain reaction on single human blastomeres. Set ting: Bad quality embryos from IVF analyzed in an academic research ge netics lab. Interventions: Patients underwent standard IVF procedures as infertility treatment. Results: Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated. Conclusion: The tech nique of PCR on single blastomeres is ready to be used in clinical pre implantation diagnosis for Tay-Sachs disease.