PURIFICATION AND CHARACTERIZATION OF A CYTOCHROME-P450 ENZYME FROM PIG-LIVER, CATALYZING THE PHENOL OXIDATIVE COUPLING OF (R)-RETICULINE TOSALUTARIDINE, THE CRITICAL STEP IN MORPHINE BIOSYNTHESIS

Authors
AMANN T ROOS PH HUH H ZENK MH
Citation
T. Amann et al., PURIFICATION AND CHARACTERIZATION OF A CYTOCHROME-P450 ENZYME FROM PIG-LIVER, CATALYZING THE PHENOL OXIDATIVE COUPLING OF (R)-RETICULINE TOSALUTARIDINE, THE CRITICAL STEP IN MORPHINE BIOSYNTHESIS, Heterocycles, 40(1), 1995, pp. 425-440
Citations number
31
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Heterocycles → ACNP
ISSN journal
03855414
Volume
40
Issue
1
Year of publication
1995
Pages
425 - 440
Database
ISI
SICI code
0385-5414(1995)40:1<425:PACOAC>2.0.ZU;2-1
Abstract
The cytochrome P450 enzyme catalyzing the conversion of (R)-reticuline to salutaridine in the presence of cytochrome c reductase, NADPH and O-2 was purified to homogeneity from pig liver and shown to be a highl y regio- and stereoselective enzyme. This finding supports the view th at the occurrence of morphine in mammals is of endogenous and not of d ietary origin.