ARABINOGALACTAN PROTEINS IN MAIZE COLEOPTILES - DEVELOPMENTAL RELATIONSHIP TO CELL-DEATH DURING XYLEM DIFFERENTIATION BUT NOT TO EXTENSION GROWTH

The spatial and temporal expression of arabinogalactan proteins (AGPs) in the coleoptile of maize seedlings was investigated with monoclonal antibodies (MAC207, JIM13, JIM14) raised against particular AGP epito pes in carrot. MAC207 binds to a buffer-soluble AGP fraction of 90-210 kDa that also reacts with beta-glucosyl Yariv reagent and the lectin RCA(120). Immunogold-labelling showed that the MAC207 epitope is exclu sively localized in the plasma membrane. JIM13 binds to a 120 kDa comp onent of the buffer-soluble AGP fraction localized in the plasma membr ane of future sclerenchyma cells and secondary-wall thickenings of fut ure tracheids of vascular bundles. JIM14 binds to a 50 kDa component o f the salt-extractable fraction from cell walls localized in the inner most wall layer of sclerenchyma cells. These AGP epitopes demonstrate different temporal expression patterns which do not correlate with ext ension growth. Auxin had no effect on the amount of soluble AGP from c oleoptile sections, containing the growth-controlling epidermis but no vascular bundles, as measured by crossed electrophoresis. Moreover, i ncorporation of radioactive arabinose, galactose or proline into this fraction was not stimulated by auxin. These results contradict the hyp othesis that AGPs function as wall-loosening agents in auxin-mediated extension growth. The results are compatible with the notion that AGPs serve as developmental markers defining particular features of future cell differentiation. The specific association of the epitopes recogn ized by JIM13 and JIM14 with disintegrating cells suggests that the re lated AGPs identify those cells of the coleoptile which are committed to programmed cell death.