PURIFICATION OF RECOMBINANT HUMAN COAGULATION FACTOR-II AND FACTOR-IXAND PROTEIN-S EXPRESSED IN RECOMBINANT VACCINIA VIRUS-INFECTED VERO CELLS

Recombinant human coagulation factors II and IX and recombinant human protein S were expressed by the Vaccinia virus expression system in Ve ro cells and isolated from the serum-free media. All three recombinant proteins were purified by the same universal strategy. After concentr ation by anion exchange chromatography, recombinant coagulation factor s were purified by anion exchange filtration in the presence of Ca2+. The addition of Ca2+ to the vitamin K-dependent coagulation factors mo dified their physico-chemical behavior during anion exchange chromatog raphy and resulted in the separation of the recombinant coagulation pr oteins from contaminating proteins.