ODONTOBLAST PROCESSES IN DENTIN REVEALED BY FLUORESCENT DI-I

There has been controversy about the length and structure of the odont oblast process within dentin since the earliest histologic studies of teeth. Our objective was to use the fluorescent carbocyanine dye Di-I combined with a new gelatin embedment procedure and confocal microscop y to determine the structure and extent of odontoblast processes in de veloping and mature rat teeth, injured rat molars, reparative dentin, and adult monkey teeth. We found that odontoblast processes do not ext end into outer dentin or to the dentin-enamel junction except during e arly stages of development. Those in innervated regions of crown are l ong and straight, whereas those in roots are extensively branched and shorter. Cavity injury to crown dentin caused odontoblast fragments to be aspirated into outer dentin. In reparative dentin the odontoblast processes were branched and similar to those in roots. We used photoco nversion and electron microscopy to show that Di-I fills the entire od ontoblast after gelatin embedment, including the cytoplasm. This is a different type of carbocyanine staining from any previously reported, and it also stains other cells in adjacent hard tissues such as bone a nd cementum. The Di-I-gelatin method is a new way to use carbocyanine dyes. It has enabled us to solve a long-standing controversy about the histology of teeth, and it should be useful for many other studies of cell structure,