CHARACTERIZATION OF THE GENOMIC STRUCTURE, PROMOTER REGION, AND A TETRANUCLEOTIDE REPEAT POLYMORPHISM OF THE HUMAN NEUROTENSIN RECEPTOR GENE

Citation
F. Le et al., CHARACTERIZATION OF THE GENOMIC STRUCTURE, PROMOTER REGION, AND A TETRANUCLEOTIDE REPEAT POLYMORPHISM OF THE HUMAN NEUROTENSIN RECEPTOR GENE, The Journal of biological chemistry, 272(2), 1997, pp. 1315-1322
Citations number
52
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
272
Issue
2
Year of publication
1997
Pages
1315 - 1322
Database
ISI
SICI code
0021-9258(1997)272:2<1315:COTGSP>2.0.ZU;2-D
Abstract
In the present study, we have cloned the human neurotensin receptor (N TR) gene, determined its structure, demonstrated that its promoter is functional in transfection experiments, and identified the start site of transcription and a tetranucleotide repeat polymorphism that locate s at less than 3 kilobase pairs from the gene. The gene contains three introns, all in the coding regions, Several differences in genomic cl ones and previously characterized cDNA sequences are reconciled, The 5 ' regulatory region, which is rich in presumptive transcription factor s, can drive luciferase expression in transfected CHO-K1 cells, Stepwi se 5' deletions identify a positive modulator between -782 and -1309 a nd a negative modulator between -1309 and -1563. Southern blot analyse s demonstrate a single copy gene for the NTR, The tetranucleotide repe at polymorphism is highly informative with at least 23 alleles and mig ht serve as a very useful marker for genetic study of the relationship between the NTR and neuropsychiatric disorders.