PURIFICATION OF MICROSOMAL NATIVE CYTOCHROME-B(5) FROM POTATO-TUBERS (SOLANUM-TUBEROSUM L)

Authors
DAVIOUD E LEDERER F KADER JC
Citation
E. Davioud et al., PURIFICATION OF MICROSOMAL NATIVE CYTOCHROME-B(5) FROM POTATO-TUBERS (SOLANUM-TUBEROSUM L), Journal of plant physiology, 145(1-2), 1995, pp. 181-184
Citations number
13
Categorie Soggetti
Plant Sciences
Journal title
Journal of plant physiology → ACNP
ISSN journal
01761617
Volume
145
Issue
1-2
Year of publication
1995
Pages
181 - 184
Database
ISI
SICI code
0176-1617(1995)145:1-2<181:POMNCF>2.0.ZU;2-Z
Abstract
Detergent-solubilized cytochrome b(5) from potato tuber microsomes was purified in its native form to a specific content of 25.8 nmol per mg of protein, by using a combination of conventional and high performan ce (FPLC) chromatography. The highly purified cytochrome bs has an app arent molecular mass of 17 kDa on SDS-PAGE.