STUDY OF PROTECTIVE EFFECT OF HIGHLY POLYMERIZED DEOXYRIBONUCLEOTIDES(HPDR) AGAINST LIPID-PEROXIDATION

The aim of this work is to study the inhibition of lipid peroxidation and the free radical scavenging properties of HPDR, extraction product of salmon milt containing 95% of HPDR. Micelles and cell cultures wer e used to study the effect of HPDR on the auto-oxidation rare of linol eic acid and arachidonic acid. The effect of HPDR on the anti-oxidant and pro-oxidant activity of vitamin E (alpha-tocopherol) was also inve stigated in this system. Rat hepatocytes in primary culture were used as a second model. Lipid peroxidation induced by the addition of iron was assessed by malonic dialdehyde production in cells and in media. A n electron spin resonance study of the capacity of HPDR to scavenge th e hydroxyl radical was carried out as the identification of 8-hydroxyd eoxyguanosine, resulting from hydroxyl radical addition on HPDR.