HEAVY-METAL DETOXIFICATION BY TRIMERCAPTO-S-TRIAZINE (TMT) AS EVALUATED BY A BACTERIAL ENZYME ASSAY

Citation
Gm. Ayoub et al., HEAVY-METAL DETOXIFICATION BY TRIMERCAPTO-S-TRIAZINE (TMT) AS EVALUATED BY A BACTERIAL ENZYME ASSAY, Environmental toxicology and chemistry, 14(2), 1995, pp. 193-196
Citations number
16
Categorie Soggetti
Toxicology,"Environmental Sciences",Chemistry
ISSN journal
07307268
Volume
14
Issue
2
Year of publication
1995
Pages
193 - 196
Database
ISI
SICI code
0730-7268(1995)14:2<193:HDBT(A>2.0.ZU;2-D
Abstract
We compared trimercapto-s-triazine (TMT) to ethylenediaminetetraacetat e (EDTA) for detoxification of five selected heavy metals as part of a n effluent fractionation procedure. Toxicity was assessed using a micr obial microplate assay, based on the MetPAD(TM) test kit. The affinity of TMT to four of the metals (Cd, Cu, Hg, Pb) was on the same order o f magnitude as EDTA, as indicated by minimum mole ratios of ligand to metal required for detoxification. The TMT was ineffective against Zn. Both TMT and EDTA were toxic (causing more than 30% inhibition) to th e test bacterial enzyme at concentrations of 3,000 muM or greater. The range of TMT concentrations that detoxified the metals was broader th an that of EDTA. Stimulation of enzyme activity by EDTA could complica te interpretation of test data, a problem not shared by TMT.